| Plasmid Name | pLVX-TRE3G | Promoter | TRE3G |
|---|---|---|---|
| Replicon | pUC | Copy Number | high |
| Competent Cell | Stbl3 | Culture Temp | 37°C |
| Forward Primer | CPPT-F：gcaggggaaagaatagtagac | Reverse Primer | pmscv-3：ACGTGCTACTTCCATTTGTC |
| Induction | Tetracycline induction | Storage Temp | 37°C |
1.For Research Use Only
All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.
2.Plasmid Sequence and Experimental Performance
Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.
3.Sequence Verification of Certain Vector Products
For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.
4.After-Sales Period for Small-Pack Plasmid Products
The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.
5.Research-Related Risks and Limitation of Liability
Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes.
6.Citation in Chinese Publications
For publications in Chinese, please cite the product as follows:
7.Product Name (Product Item Number) was provided by the MiaoLing Plasmid Platform.
Citation in English Publications
For publications in English, please cite the product as follows:
“Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
5' LTR 1..634
/note="5'长末端重复序列（LTR）源自1型人类免疫缺陷病毒"
HIV-1 psi 681..806
/note="1型人类免疫缺陷病毒的包装信号"
RRE 1303..1536
/note="1型人类免疫缺陷病毒的Rev反应元件（RRE）允许Rev依赖的mRNA从核内运输到细胞质中。"
cPPT/CTS 2028..2144
/note="1型人类免疫缺陷病毒的中心多嘌呤道和中心终止序列（缺少第一个T）。"
TRE3GV promoter 2209..2555
/note="第三代四环素响应型启动子，可通过Tet-On 3G的结合而被激活；针对逆转录病毒和慢病毒载体进行了优化。"
tet operator 2214..2232
/gene="tetO"
/bound_moiety="tetracycline repressor TetR"
/note="细菌操作子O2，用于tetR和tetA基因。"
tet operator 2250..2268
/gene="tetO"
/bound_moiety="tetracycline repressor TetR"
/note="细菌操作子O2，用于tetR和tetA基因。"
tet operator 2286..2304
/gene="tetO"
/bound_moiety="tetracycline repressor TetR"
/note="细菌操纵子O2，用于tetR和tetA基因。"
tet operator 2322..2340
/gene="tetO"
/bound_moiety="tetracycline repressor TetR"
/note="细菌操作子O2，用于tetR和tetA基因。"
tet operator 2358..2376
/gene="tetO"
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
tet operator 2394..2412
/gene="tetO"
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
tet operator 2430..2448
/gene="tetO"
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
MCS 2556..2599
/note="多克隆位点"
PGK promoter 2604..3103
/note="小鼠磷酸甘油酸激酶1启动子"
PuroR 3124..3723
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/note="赋予对普鲁霉素的抗性"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
WPRE 3741..4329
/note="土拨鼠肝炎病毒转录后调控元件"
3' LTR 4537..5170
/note="1型人类免疫缺陷病毒的3'长末端重复序列"
ori complement(5701..6286)
/direction=LEFT
/note="高拷贝数 ColE1/pMB1/pBR322/pUC 复制起点"
AmpR complement(6457..7317)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="赋予对氨苄青霉素、卡那霉素和相关抗生素的抗性。"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
AmpR promoter complement(7318..7422)
/gene="bla"
SV40 poly(A) signal 7470..7604
/note="SV40 polyA信号" 