| Plasmid Name | pLP2 | Alternative Names | DB00027;pLP-2;pRev |
|---|---|---|---|
| Promoter | RSV | Replicon | pUC |
| Copy Number | high | Competent Cell | DH5a |
| Culture Temp | 37°C | Forward Primer | pTRE2.F：TCGTTTAGTGAACCGTCAGA |
| Reverse Primer | M13F：TGTAAAACGACGGCCAGT | Storage Temp | 37°C |
1.For Research Use Only
All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.
2.Plasmid Sequence and Experimental Performance
Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.
3.Sequence Verification of Certain Vector Products
For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.
4.After-Sales Period for Small-Pack Plasmid Products
The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.
5.Research-Related Risks and Limitation of Liability
Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes.
6.Citation in Chinese Publications
For publications in Chinese, please cite the product as follows:
7.Product Name (Product Item Number) was provided by the MiaoLing Plasmid Platform.
Citation in English Publications
For publications in English, please cite the product as follows:
“Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
RSV promoter 1..271
/note="禽类肉瘤病毒增强子/启动子"
Rev 391..741
/codon_start=1
/translation="MAGRSGDSDEDLLKAVRLIKFLYQSNPPPNPEGTRQARRNRRRRW
RERQRQIHSISERILSTYLGRSAEPVPLQLPPLERLTLDCNEDCGTSGTQGVGSPQILV
ESPTILESGAKE"
primer_bind complement(825..841)
/note="通用测序引物，多个类似变体之一"
M13F complement(825..841)
HIV-1 LTR polyA signal 850..971
f1 ori 1173..1628
/direction=RIGHT
/note="f1 噬菌体复制起点；箭头指示(+)链合成方向"
AmpR promoter 1910..2014
/gene="bla"
AmpR 2015..2875
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="赋予对氨苄西林、卡那西林和相关抗生素的抗性。"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
ori 3046..3634
/direction=RIGHT
/note="高拷贝数 ColE1/pMB1/pBR322/pUC 复制起点"
CAP binding site 3922..3943
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
lac promoter 3958..3988
/note="promoter for the E. coli lac operon"
lac operator 3996..4012
/bound_moiety="lac repressor encoded by lacI"
/note="lac阻遏蛋白结合到lac操纵子上以抑制大肠杆菌中的转录。这种抑制可以通过添加乳糖或异丙基-β-D-硫代半乳糖苷（IPTG）来解除。"
primer_bind 4020..4036
/note="通用测序引物，多个类似变体之一"
M13R 4020..4036 