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psPAX2

产品货号 P0261
产品别称 DB00001;Gag-Pol-Rev,慢病毒2代3质粒包装质粒
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价格： ¥390.00
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基本信息

产品简介

产品声明

  1. For Research Use Only
    All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.
  2. Plasmid Sequence and Experimental Performance
    Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.
  3. Sequence Verification of Certain Vector Products
    For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.
  4. After-Sales Period for Small-Pack Plasmid Products
    The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.
  5. Research-Related Risks and Limitation of Liability
    Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes.
  6. Citation in Chinese Publications
    For publications in Chinese, please cite the product as follows:
    产品名称（产品货号）由淼灵质粒平台提供。
  7. Citation in English Publications
    For publications in English, please cite the product as follows:
    “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”

相关论文

1、 Zhongkai Wu;Lilong Zhang;Xinyi Li;Li Liu;Tianrui Kuang;Zhendong Qiu;Wenhong Deng;Weixing Wang,The prognostic significance and potential mechanism of DBF4 zinc finger in hepatocellular carcinoma,2024-4-22,DOI:10.1038/s41598-024-60342-w,(IF:3.8)
2、 Qianwu Huang;Jun Lv;Ting Dong;Haijun Liu;Lei Xu;Mingcai Wu,Cryptochrome 1 alleviates the antiproliferative effect of isoproterenol on human gastric cancer cells,2020-6-1,DOI:10.1177/1559325820939022,(IF:2.3)
3、 Haixiao Duan; Ershuai Zhang; Ge Ren; Yining Cheng; Binfeng Yang; Lirong Liu; Normand Jolicoeur; Han Hu; Yan Xu; Binlei Liu,Exploring immune evasion of SARS-CoV-2 variants using a pseudotyped system,2024-4-18,DOI:10.1016/j.heliyon.2024.e29939,(IF:3.4)
4、 Zhongkai Wu;Lilong Zhang;Xinyi Li;Li Liu;Tianrui Kuang;Zhendong Qiu;Wenhong Deng;Weixing Wang,The prognostic significance and potential mechanism of DBF4 zinc finger in hepatocellular carcinoma,2024-4-22,DOI:10.1038/s41598-024-60342-w,(IF:3.8)
5、 Xiaoxue Zhang; Liujun He; Yong Li; Yifei Qiu; Wujing Hu; Wanying Lu; Huihui Du; Donglin Yang,Compound 225# inhibits the proliferation of human colorectal cancer cells by promoting cell cycle arrest and apoptosis induction,2023-7-7,DOI:10.3892/or.2024.8729,(IF:2.5)
6、 Bo Zhai; Yang Zhou; Hui Yan; Qiang Zhou; Penggao Wang; Fang Yang; Ziqiao Yuan; Yunjiang Zhou,CCNB1IP1 stabilizes MYCN through suppression of Fbxw7-mediated degradation and facilitates the growth of MYCN-amplified neuroblastoma,2022-7-28,DOI:10.21203/rs.3.rs-1876730/v1,(IF:preprint)
7、 Zihao Mai; Han Sun; Fangfang Yang; Mengyan Du; Xuecheng Cheng; Hongce Chen; Beini Sun; Junlin Wen; Xiaoping Wang; Tongsheng Chen,Bad is essential for Bcl-xL-enhanced Bax shuttling between mitochondria and cytosol,2022-12-29,DOI:10.1016/j.biocel.2022.106359,(IF:5.6)
8、 Hui Xiang; Miao Wang; Yi-Fang Chen; Hao-Ming Wu; Ming-Ge Li; Lei Guo; Ying-Yi Zhang; He-Zhe Lu,Regulation of cancer cell Ferroptosis by PTRF/Cavin-1,2024-1-24,DOI:10.1080/10715762.2024.2386457,(IF:3.6)
9、 Peng Xu; Siyang Li; Ke Liu; Rui Fan; Fahui Liu; Haoxuan Zhang; Donghua Liu; Dongyan Shen,Downregulation of dermatopontin in cholangiocarcinoma cells suppresses CCL19 secretion of macrophages and immune infiltration,2024-2-1,DOI:10.1007/s00432-023-05532-1,(IF:2.7)
10、 Donghu Zhou;Siyu Jia;Liping Yi;Zhen Wu;Yi Song;Bei Zhang;Yanmeng Li;Xiaoxi Yang;Anjian Xu;Xiaojin Li;Wei Zhang;Weijia Duan;Zhenkun Li;Saiping Qi;Zhibin Chen;Qin Ouyang;Jidong Jia;Jian Huang;Xiaojuan Ou;Hong You,Identification of potential modifier genes in Chinese patients with Wilson disease,2025-2-21,DOI:10.1093/mtomcs/mfac024,(IF:2.9)
11、 Xue-Dong Yu;Qi-Ya Zhang;Fei Ke,Crispr/Cas13d Mediated Inhibition of Siniperca Chuatsi Rhabdovirus Infection in Fish Cells,2025-02-27,DOI:,(IF:3.9)
12、 Yang Zhou;Hui Yan;Qiang Zhou;Penggao Wang;Fang Yang;Ziqiao Yuan;Qianming Du;Bo Zhai,CCNB1IP1 prevents ubiquitination-mediated destabilization of MYCN and potentiates tumourigenesis of MYCN-amplificated neuroblastoma,2023-7-5,DOI:10.1002/ctm2.1328,(IF:7.9)
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产品文件

4032_PCMV-R_TSS20250623-027-06040_H04.ab1 P0261_全序列图谱.dna psPAX2 质粒说明书.pdf

质粒元件

intron 654..1670 /note="鸡β-肌动蛋白和兔β-球蛋白内含子之间的嵌合体" HIV-1 gag 1776..3278 /codon_start=1 /gene="gag" /product="gag protein from human immunodeficiency virus 1" /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ" HIV-1 pol 3071..6082 /codon_start=1 /gene="pol" /product="pol protein from human immunodeficiency virus 1" /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ MAGDDCVASRQDED" cPPT/CTS 5767..5884 /note="1型人类免疫缺陷病毒的中心多嘌呤道和中心终止序列" RRE 6656..6889 /note="1型人类免疫缺陷病毒的Rev反应元件（RRE）允许Rev依赖的mRNA从核内运输到细胞质中。" CDS 7074..7118 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /note="被2H10单链骆驼纳米抗体识别" /translation="KNEQELLELDKWASL" NES 7412..7438 /codon_start=1 /product="nuclear export signal from the HIV Rev protein (Fischer et al., 1995)" /translation="LPPLERLTL" beta-globin poly(A) signal 7788..7843 /note="兔β-珠蛋白多聚腺苷酸信号（Gil和Proudfoot，1987）" primer_bind complement(8204..8220) /note="通用测序引物，多个类似变体之一" lac operator 8228..8244 /bound_moiety="lac repressor encoded by lacI" /note="lac阻遏蛋白结合到lac操纵子上以抑制大肠杆菌中的转录。这种抑制可以通过添加乳糖或异丙基-β-D-硫代半乳糖苷（IPTG）来解除。" lac promoter complement(8252..8282) /note="大肠杆菌乳糖操纵子的启动子" CAP binding site 8297..8318 /bound_moiety="E. coli catabolite activator protein" /note="CAP结合激活了cAMP存在的条件下的转录。" SV40 promoter 8377..8572 /note="SV40早期启动子" SV40 ori 8423..8558 /note="SV40复制起点" SV40 poly(A) signal 8578..8712 /note="SV40 polyA信号" ori complement(8951..9539) /direction=LEFT /note="高拷贝数 ColE1/pMB1/pBR322/pUC 复制起点" AmpR complement(9710..10570) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" Cleavage site after base 10501" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" AmpR promoter complement(10571..10675) /gene="bla" CMV enhancer join(10706..10709,1..376) /note="人类巨细胞病毒即刻早期增强子"
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