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pmirGLO

产品货号 P0198
产品别称 DB00009;pmir-GLO
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价格: ¥280.00
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基本信息

产品简介

产品声明

  1. For Research Use Only
    All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.
  2. Plasmid Sequence and Experimental Performance
    Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.
  3. Sequence Verification of Certain Vector Products
    For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.
  4. After-Sales Period for Small-Pack Plasmid Products
    The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.
  5. Research-Related Risks and Limitation of Liability
    Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes.
  6. Citation in Chinese Publications
    For publications in Chinese, please cite the product as follows:
    产品名称(产品货号)由淼灵质粒平台提供。
  7. Citation in English Publications
    For publications in English, please cite the product as follows:
    “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”

相关论文

1、 李阳宇;景丽;冰鹏;鹏蔡;百林赵;英陈;海玲朱,RETRACTED ARTICLE: CircASXL1 Knockdown Restrains Hypoxia-Induced DDP Resistance and NSCLC Progression by Sponging miR-206,2021-06-28,DOI:10.2147/CMAR.S276964,(IF:2.5)
2、 Cuiyun Zhou;Xiaoqiong Huang;Xia Li;Yan Xiong,Circular RNA erythrocyte membrane protein band 4.1 assuages ultraviolet irradiation-induced apoptosis of lens epithelial cells by stimulating 5’-bisphosphate nucleotidase 1 in a miR-24-3p-dependent manner,2021-8-12,DOI:10.1080/21655979.2021.1990196,(IF:4.2)
3、 Zhenzhu Zhu;Liuyue Liao;Mingwei Gao;Qin Liu,Garlic-derived exosome-like nanovesicles alleviate dextran sulphate sodium-induced mouse colitisviathe TLR4/MyD88/NF-κB pathway and gut microbiota modulation,2023-7-14,DOI:10.1039/d3fo01094e,(IF:5.1)
4、 Jing Wang;Na Huo;Chuan Cai;Yu Zhang;Rui Xiao,Circ_0087199 depletion attenuates lipopolysaccharides-induced human periodontal ligament cell injury through the miR-527/TLR4 axis,2023-7-14,DOI:10.1002/iid3.1153,(IF:3.1)
5、 Wei Chen;Xiao Yu;Ning Wang;Jiangpeng Jing;Ruichun Li;Minxue Lian,Circ_RPPH1 regulates glioma cell malignancy by binding to miR-627-5p/miR-663a to induce SDC1 expression,2022-3-25,DOI:10.1007/s11011-022-00965-y,(IF:3.2)
6、 Weimin Li;Qingqin Ma;Quan Liu;Peng Yan;Xin Wang;Xiaojun Jia,Circ-VPS18 knockdown enhances TMZ sensitivity and inhibits glioma progression by MiR-370/RUNX1 Axis,2020-11-13,DOI:10.1007/s12031-020-01749-8,(IF:2.8)
7、 Xiangjun Yi;Xuan Chen;Zhenbin Li,miR-200c targeting GLI3 inhibits cell proliferation and promotes apoptosis in non-small cell lung cancer cells,2023-9-26,DOI:10.1097/MD.0000000000039658,(IF:1.4)
8、 Shujing Feng; Hao Zhou; Xingzuan Lin; Siyuan Zhu; Huifang Chen; Han Zhou; Ru Wang; Peng Wang; Xiexiang Shao; Jianhua Wang; Hao Zhou,Exercise promotes skeletal muscle growth in adolescents via modulating Mettl3-mediated m6A methylation of MyoD in muscle satellite cells,2024-6-5,DOI:10.1186/s11658-024-00670-x,(IF:9.2)
9、 Jianjun Wang;Kunxian Yang;Junyu Cao;Li Li,Knockdown of circular RNA septin 9 inhibits the malignant progression of breast cancer by reducing the expression of solute carrier family 1 member 5 in a microRNA-149-5p-dependent manner,2021-8-6,DOI:10.1080/21655979.2021.2000731,(IF:4.2)
10、 Xi Yang;Qunxiong Zhang;Bugao Guan,Circ_0110805 knockdown enhances cisplatin sensitivity and inhibits gastric cancer progression by miR-299-3p/ENDOPDI axis,2020-11-6,DOI:10.2147/OTT.S279563,(IF:2.7)

产品文件

质粒元件

polyA_signal 115..236 /note="SV40 polyA信号" pSV40 481..838 /note="SV40增强子和早期启动子" SV40 ori 689..824 /note="SV40复制起点" hRluc 889..1821 /codon_start=1 /product="Renilla luciferase" /note="人类密码子优化" /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ" Neo 1873..2664 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="赋予对新霉素、卡那霉素和G418(金霉素)的抗性。" /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" poly(A) 2728..2776 /note="人工合成polyA信号" pAmp 2932..3036 /gene="bla" Amp 3037..3897 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="赋予对氨苄青霉素、卡那霉素和相关抗生素的抗性。" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" ori complement(4055..4643) /direction=LEFT /note="高拷贝数 ColE1/pMB1/pBR322/pUC 复制起点" cer 4759..5042 /note="源自ColE1的重组位点,有助于维持质粒为单体形式。" pPGK 5099..5598 /note="小鼠磷酸甘油酸激酶1启动子" Luc 5645..7297 /codon_start=1 /product="firefly luciferase" /note="人工合成的luc2版本的荧光素酶基因" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
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