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pCMV-Tag 2B

产品货号 P0175
产品别称
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价格: ¥480.00
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基本信息

产品简介

产品声明

  1. For Research Use Only
    All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.
  2. Plasmid Sequence and Experimental Performance
    Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.
  3. Sequence Verification of Certain Vector Products
    For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.
  4. After-Sales Period for Small-Pack Plasmid Products
    The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.
  5. Research-Related Risks and Limitation of Liability
    Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes.
  6. Citation in Chinese Publications
    For publications in Chinese, please cite the product as follows:
    产品名称(产品货号)由淼灵质粒平台提供。
  7. Citation in English Publications
    For publications in English, please cite the product as follows:
    “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”

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质粒元件

CMV enhancer 67..370 /note="人类巨细胞病毒即刻早期增强子" CMV promoter 371..574 /note="人类巨细胞病毒(CMV)即刻早期启动子" T3 promoter 620..638 /note="T3 噬菌体 RNA 聚合酶的启动子" CDS 682..705 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /translation="DYKDDDDK" primer_bind complement(753..769) /note="common sequencing primer, one of multiple similar variants" T7 promoter complement(826..844) /note="promoter for bacteriophage T7 RNA polymerase" SV40 poly(A) signal 1118..1239 /note="SV40 polyadenylation signal" f1 ori complement(1246..1701) /direction=LEFT /note="f1 噬菌体复制起点;箭头指示(+)链合成方向" AmpR promoter 1728..1832 /gene="bla" SV40 promoter 1834..2191 /note="SV40 增强子和早期启动子" SV40 ori 2042..2177 /note="SV40复制起点" NeoR/KanR 2226..3020 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="赋予对新霉素、卡那霉素和G418(金霉素)的抗性。" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" HSV TK poly(A) signal 3252..3299 /note="单纯疱疹病毒胸苷激酶polyA信号(Cole和Stacy,1985)" ori 3628..4216 /direction=RIGHT /note="高拷贝数 ColE1/pMB1/pBR322/pUC 复制起点"
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