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pRSFDuet-1

产品货号 P0051
产品别称 pRSFDuet1
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价格: ¥480.00
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基本信息

产品简介

产品声明

  1. For Research Use Only
    All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.
  2. Plasmid Sequence and Experimental Performance
    Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.
  3. Sequence Verification of Certain Vector Products
    For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.
  4. After-Sales Period for Small-Pack Plasmid Products
    The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.
  5. Research-Related Risks and Limitation of Liability
    Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes.
  6. Citation in Chinese Publications
    For publications in Chinese, please cite the product as follows:
    产品名称(产品货号)由淼灵质粒平台提供。
  7. Citation in English Publications
    For publications in English, please cite the product as follows:
    “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”

相关论文

产品文件

质粒元件

lac operator 3..27 /bound_moiety="lac repressor encoded by lacI" /note="lac阻遏蛋白结合到lac操纵子上以抑制大肠杆菌中的转录。这种抑制可以通过添加乳糖或异丙基-β-D-硫代半乳糖苷(IPTG)来解除。" 6xHis 83..100 /codon_start=1 /product="6xHis affinity tag" /translation="HHHHHH" MCS 106..168 primer_bind complement(189..208) primer_bind 189..208 T7 promoter 214..232 /note="1型人类免疫缺陷病毒RNA聚合酶启动子" MCS 297..359 CDS 366..410 /codon_start=1 /product="affinity and epitope tag derived from pancreatic ribonuclease A" /translation="KETAAAKFERQHMDS" primer_bind complement(448..467) terminator 462..509 /note="1型人类免疫缺陷病毒转录终止子,用于噬菌体T7 RNA聚合酶" KanR complement(739..1554) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /note="赋予细菌对卡那霉素的抗性或真核生物对G418(Geneticin(R))的抗性。" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" AmpR promoter complement(1555..1646) /gene="bla" RSF ori complement(1662..2411) /direction=LEFT /note="含有RSF 1030复制起点的质粒可以在含有兼容复制起点的额外质粒的大肠杆菌细胞中进行扩增。" lacI complement(2607..3689) /codon_start=1 /gene="lacI" /product="lac repressor" /note="lac阻遏蛋白结合到lac操纵子上以抑制大肠杆菌中的转录。这种抑制可以通过添加乳糖或异丙基-β-D-硫代半乳糖苷(IPTG)来解除。" /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" lacI promoter complement(3690..3767) /gene="lacI" primer_bind 3770..3788 T7 promoter join(3813..3829,1..2) /note="1型人类免疫缺陷病毒RNA聚合酶启动子"
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